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- Title
ION CHANNELS - MEMBRANE TRANSPORT - INTEGRATIVE PHYSIOLOGY Regulation of gene expression by dietary Ca<sup>2+</sup> in kidneys of 25-hydroxyvitamin D<sub>3</sub>-1α-hydroxylase knockout mice.
- Authors
Hoenderop, Joost G.J.; Chon, Helena; Gkika, Dimitra; Bluyssen, Hans A.R.; Holstege, Frank C.P.; St-Arnaud, Rene; Braam, Branko; Bindels, Rene J.M.
- Abstract
Regulation of gene expression by dietary Ca2+ in kidneys of 25-hydroxyvitamin d3-1α-hydroxylase knockout mice. Background. Pseudovitamin D deficiency rickets (PDDR) is an autosomal disease, characterized by undetectable levels of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), rickets and secondary hyperparathyroidism. Mice in which the 25-hydroxyvitamin D3-1α-hydroxylase (1α-OHase) gene was inactivated, presented the same clinical phenotype as patients with PDDR. Methods. cDNA Microarray technology was used on kidneys of 1α-OHase knockout mice to study the expression profile of renal genes in this Ca2+-related disorder. Genome wide molecular events that occur during the rescue of these mice by high dietary Ca2+ intake were studied by the use of 15K cDNA microarray chips. Results. 1α-OHase knockout mice fed a normal Ca2+ diet developed severe hypocalcemia, rickets and died with an average life span of 12 ± 2 weeks. Intriguingly, 1α-OHase−/− mice supplemented with an enriched Ca2+ diet were normocalcemic and not significantly different from wild-type mice. Inactivation of the 1α-OHase gene resulted in a significant regulation of ± 1000 genes, whereas dietary Ca2+ supplementation of the 1α-OHase−/− mice revealed ± 2000 controlled genes. Interestingly, 557 transcripts were regulated in both situations implicating the involvement in the dietary Ca2+-mediated rescue mechanism of the 1α-OHase−/− mice. Conspicuous regulated genes encoded for signaling molecules like the PDZ-domain containing protein channel interacting protein, FK binding protein type 4, kinases, and importantly Ca2+ transporting proteins including the Na+-Ca2+ exchanger, calbindin-D28K and the Ca2+ sensor calmodulin. Conclusion. Dietary Ca2+ intake normalized disturbances in the Ca2+ homeostasis due to vitamin D deficiency that were accompanied by the regulation of a subset of renal genes, including well-known renal Ca2+ transport protein genes, but also genes not previously identified as playing a role in renal Ca2+ handling.
- Subjects
RICKETS; VITAMIN deficiency; VITAMIN D; GENE expression; NUTRITIONALLY induced diseases; CALCIUM; KIDNEYS
- Publication
Kidney International, 2004, Vol 65, Issue 2, p531
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1111/j.1523-1755.2004.00402.x