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- Title
Porcine CD3ε: its characterization, expression and involvement in activation of porcine T lymphocytes.
- Authors
Kirkham, P. A.; Takamatsu, H.; Yang, H.; Parkhouse, R. M. E.
- Abstract
The cloning, characterization and expression of porcine CD3∊ and establishment of its role in T-cell activation using an anti-porcine CD3∊ monoclonal antibody, as described here, provides a first step towards a greater understanding of the porcine immune response. Porcine CD3∊ was cloned from a porcine T-cell cDNA library by polymerase chain reaction and found to have up to 72% identity with other CD3∊ chains, retaining all the necessary primary structural motifs for correct functioning of porcine CD3∊. When expressed in COS7 cells porcine CD3∊ was an intracellularly localized, monomeric 23 000 MW protein exhibiting no evidence of N-glycosylation. A monoclonal antibody, PPT3, recognized expressed porcine CD3∊ and activated porcine T cells as demonstrated by stimulation of calcium mobilization, an increase in protein tyrosine phosphorylation and proliferation. These results further reaffirm and identify CD3∊ as an important cell surface protein involved in signal transduction of activation signals in porcine T cells.
- Subjects
T cells; MONOCLONAL antibodies; DNA; PHOSPHORYLATION; CELL proliferation; IMMUNOGLOBULINS
- Publication
Immunology, 1996, Vol 87, Issue 4, p616
- ISSN
0019-2805
- Publication type
Article
- DOI
10.1046/j.1365-2567.1996.498566.x