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- Title
Identification of a Novel, Membrane-Associated Neuronal Kinase, Cyclin-Dependent Kinase 5/p35-Regulated Kinase.
- Authors
Kesavapany, Sashi; Kwok-Fai Lau; Ackerley, Steven; Banner, Steven J.; Shemilt, Stephen J. A.; Cooper, Jonathan D.; Leigh, P. Nigel; Shaw, Christopher E.; McLoughlin, Declan M.; Miller, Christopher C. J.
- Abstract
Here we characterize a novel neuronal kinase, cyclin-dependent kinase 5 (cdk5)/p35-regulated kinase (cprk). Cprk is a member of a previously undescribed family of kinases that are predicted to contain two N-terminal membrane-spanning domains and a long C terminus, which harbors a dual-specificity serine/threonine/tyrosine kinase domain. Cprk was isolated in a yeast two-hybrid screen using the neuronal cdk5 activator p35 as "bait." Cprk interacts with p35 in the yeast-two hybrid system, binds to p35 in glutathione S-transferase fusion pull-down assays, and colocalizes with p35 in cultured neurons and transfected cells. In these cells, cprk is present with p35 in the Golgi apparatus. Cprk is expressed in a number of tissues but is enriched in brain and muscle and within the brain is found in a wide range of neuronal populations. Cprk displays catalytic activity in in vitro kinase assays and is itself phosphorylated by cdk5/p35. Cdk5/p35 inhibits cprk activity. Cdk5/p35 may therefore regulate cprk function in the brain.
- Subjects
CYCLIN-dependent kinases; NEURONS; PROTEIN kinases; CYTOPLASMIC filaments; AMYOTROPHIC lateral sclerosis
- Publication
Journal of Neuroscience, 2003, Vol 23, Issue 12, p4975
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.23-12-04975.2003