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- Title
Assembly of Nano‐Biocatalyst for the Tandem Hydrolysis and Reduction of p‐Nitrophenol Esters.
- Authors
de Barros, Heloise Ribeiro; Tanaka, Lívia Yukari; da Silva, Rafael Trivella Pacheco; Santiago‐Arcos, Javier; de Torresi, Susana I. Córdoba; López‐Gallego, Fernando
- Abstract
Hybrid nano‐biomaterials are exploited in the design and performance of chemo‐enzymatic cascades. In this study, lipase is immobilized from Candida antarctica fraction B (CALB) and gold nanoparticles (Au NPs) on magnetic particles coated with silica (MNP@SiO2) to stepwise hydrolyze and reduce p‐nitrophenyl esters in tandem reaction. The assembly of the two catalysts at the interface of the MNP@SiO2 particles and the temporal control of the reaction turns out to be the most determinant parameters for the cascade kinetics. When both CALB and Au NPs are co‐immobilized at the MNP@SiO2 particle, the tandem reactions take place significantly faster than when both catalysts are physically segregated by their immobilization on different MNP@SiO2 particles. Herein, it is demonstrated that the co‐immobilization of biocatalysts and nanocatalysts in solid materials creates hybrid interfaces that accelerated chemo‐enzymatic tandem reactions.
- Subjects
MAGNETIC particles; GOLD nanoparticles; ENZYMES; LIPASES; BIOCATALYSIS
- Publication
Particle & Particle Systems Characterization, 2021, Vol 38, Issue 10, p1
- ISSN
0934-0866
- Publication type
Article
- DOI
10.1002/ppsc.202100136