We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Nanoporous silica nanoparticles as biomaterials: evaluation of different strategies for the functionalization with polysialic acid by step-by-step cytocompatibility testing.
- Authors
Williams, Sina; Neumann, Anne; Bremer, Imke; Su, Yi; Dräger, Gerald; Kasper, Cornelia; Behrens, Peter
- Abstract
Nanoporous silica materials have become a prominent novel class of biomaterials which are typically applied as nanoparticles or thin films. Their large surface area combined with the rich surface chemistry of amorphous silica affords the possibility to equip this material with variable functionalities, also with several different ones on the same particle or coating. Although many studies have shown that nanoporous silica is apparently non-toxic and basically biocompatible, any surface modification may change the surface properties considerably and, therefore, the modified materials should be checked for their biocompatibility at every step. Here we report on different silane-based functionalization strategies, firstly a conventional succinic anhydride-based linker system and, secondly, copper-catalyzed click chemistry, to bind polysialic acid, a polysaccharide important in neurogenesis, onto nanoporous silica nanoparticles (NPSNPs) of MCM-41 type. At each of the different modification steps, the materials are characterized by cell culture experiments. The results show that polysialic acid can be immobilized on the surface of NPSNPs by using different strategies. The cell culture experiments show that the kind of surface immobilization has a strong influence on the toxicity of the material versus the cells. Whereas most modifications appear inoffensive, NPSNPs modified by click reactions are toxic, probably due to residues of the Cu catalyst used in these reactions.
- Subjects
BIOMATERIALS; BIOLOGICAL specimen analysis; SILICA nanoparticles; POLYSIALIC acid; NANOPOROUS materials; MCM-41 (Mesoporous material); CLICK chemistry; COPPER catalysts; LIVER cancer
- Publication
Journal of Materials Science: Materials in Medicine, 2015, Vol 26, Issue 3, p1
- ISSN
0957-4530
- Publication type
Article
- DOI
10.1007/s10856-015-5409-3