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- Title
Inactivation of the FNR protein of Escherichia coli by targeted mutagenesis in the N-terminal region.
- Authors
Spiro, S.; Guest, J. R.
- Abstract
The FNR protein of Escherichia coli is a regulatory protein that activates the transcription of its target genes in response to oxygen limitation. Site-directed mutagenesis was used to show that a 28-residue N-terminal segment containing three cysteines is essential for normal FNR function. The cysteine residue which is centrally located in the three-cysteine cluster (Cys-Ala-Ile-His-Cys-Gln-Asp-Cys) was also shown to be essential for FNR activity. Possible mechanisms by which this cysteine residue might function in the response of FNR to anaerobiosis are discussed.
- Subjects
BACTERIAL proteins; ESCHERICHIA coli; GENETIC transcription; CYSTEINE proteinases; PHYSIOLOGICAL effects of oxygen; MUTAGENESIS; ANAEROBIOSIS
- Publication
Molecular Microbiology, 1988, Vol 2, Issue 6, p701
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.1988.tb00080.x