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- Title
MADS1, a novel MADS-box protein, is involved in the response of Nicotiana benthamiana to bacterial harpin<sub>Xoo</sub>.
- Authors
Huajian Zhang; Wenjun Teng; Jingang Liang; Xinyu Liu; Haifeng Zhang; Zhengguang Zhang; Xiaobo Zheng
- Abstract
MADS-box transcription factor genes are well known for their role in floral organ and seed development. In this study, a novel MADS-box-containing gene, designated NbMADS1, was isolated from leaves of Nicotiana benthamiana. The full-length cDNA was 666 bp and encoded a putative polypeptide of 221 aa with a mass of 24.3 kDa. To assess the role of NbMADS1 in the defence response to bacterial harpinXoo, an elicitor of the hypersensitive response, a loss-of-function experiment was performed in N. benthamiana plants using virus-induced gene silencing. Analyses of electrolyte leakage revealed more extensive cell death in the control plants than in NbMADS1-silenced plants. The NbMADS1-silenced plants showed impaired harpinXoo-induced stomatal closure, decreased harpinXoo-induced production of hydrogen peroxide (H2O2) and nitric oxide (NO) in guard cells, and reduced harpinXoo-induced resistance to Phytophthora nicotianae. The compromised stomatal closure observed in the NbMADS1-silenced plants was inhibited by the application of H2O2 and sodium nitroprusside (an NO donor). Taken together, these results demonstrate that the NbMADS1-H2O2-NO pathway mediates multiple harpinXoo-triggered responses, including stomatal closure, hypersensitive cell death, and defence-related gene expression, suggesting that NbMADS1 plays an important role in regulating the response to harpinXoo in N. benthamiana plants.
- Subjects
NICOTIANA benthamiana; HARPINS; PLANT proteins; TRANSCRIPTION factors; GENE expression in plants; PLANT defenses
- Publication
Journal of Experimental Botany, 2016, Vol 67, Issue 1, p131
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erv448