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- Title
Highly efficient somatic-mutation identification using Escherichia coli mismatch-repair detection.
- Authors
Peters, Brock A.; Zhengyan Kan; Sebisanovic, Dragan; Pujara, Kanan; Zhiyong Wang; Hong, Peter; Chow, Bernard; Stinson, Jeremy; Carlton, Victoria E. H.; Pham, Thinh Q.; Stern, Howard; Waring, Paul; Hillan, Kenneth J.; Eberhard, David A.; de Sauvage, Frederic; Jianbiao Zheng; Faham, Malek; Seshagiri, Somasekar
- Abstract
The discovery of somatic mutations in cancer tissue is extremely laborious, time-consuming and costly. In an evaluation comparing mismatch repair detection (MRD) against Sanger sequencing for somatic-mutation detection, we found that MRD had a specificity of 96% and a sensitivity of 92%. Our results showed that MRD is a robust and cost-effective alternative to Sanger sequencing for identifying somatic mutations in human tumors.
- Subjects
MEDICAL care; GENETIC mutation; TUMORS; CANCER; CYSTS (Pathology); ONCOLOGY
- Publication
Nature Methods, 2007, Vol 4, Issue 9, p713
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth1081