We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Oxidative stress induces H2AX phosphorylation in human spermatozoa
- Authors
Li, Zhongxiang; Yang, Jun; Huang, Hefeng
- Abstract
Abstract: H2AX phosphorylation occurs following the induction of DNA double strand breaks (DSBs), thus collaborating with many other proteins to mediate important biological functions in somatic cells. In human spermatozoa, the present study showed that H2O2 induced H2AX phosphorylation in a time- and dose-dependent manner. Moreover, such effect could be abolished by the phosphatidylinositol 3-kinase inhibitor wortmannin. Meanwhile, the neutral comet assay also revealed DSBs production in correlation with H2AX phosphorylation assessed by flow cytometry. Besides H2AX phosphorylation, two other collaborating proteins, Rad50 and 53BP1, were also generated in spermatozoa after H2O2 exposure. However, unlike in somatic FL cells, there were no distinctive focuses, but rather a whole nuclei staining pattern of these three proteins in spermatozoa. Additionally, γH2AX (the phosphorylated form of H2AX) staining in spermatozoa persisted despite the fact of a decrease in the number of γH2AX foci in FL cells after H2O2 removal. Collectively, these results demonstrate that oxidative stress can induce H2AX phosphorylation in human spermatozoa through DSB induction, and that γH2AX may be used as a sensitive, novel marker for such DSBs. Moreover, the surveillance system involving γH2AX, Rad50, and 53BP1 in human spermatozoa cannot function effectively in DNA repair, but this system may possess other biological functions in response to DSBs.
- Subjects
GERM cells; PHOSPHORYLATION; SEMEN; CHEMICAL reactions
- Publication
FEBS Letters, 2006, Vol 580, Issue 26, p6161
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2006.10.016