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- Title
Oxidation of thioredoxin reductase in HeLa cells stimulated with tumor necrosis factor-α
- Authors
Kim, Jae-Ryong; Lee, Seon-Min; Cho, Seung-Hyun; Kim, Jung-Hyun; Kim, Byung-Hak; Kwon, Jaeyul; Choi, Cheol Yong; Kim, Yeong-Dae; Lee, Seung-Rock
- Abstract
Stimulation of cells with tumor necrosis factor-α (TNF-α) results in the increase in generation of H2O2 in mitochondria that leads to apoptosis. The effect of H2O2 produced by TNF-α on the redox status of selenocysteine (SeCys) residue essential for mitochondrial thioredoxin reductase (TrxR2) was investigated in HeLa cells. TNF-α caused accumulation of oxidized TrxR2 with a thioselenide bond. The conditional induction of SeCys-deficient TrxR2 resulted in the increased production of H2O2 and apoptosis. These results suggest that the SeCys residue of TrxR2 plays a critical role in cell survival by serving as an electron donor for Trx-II and subsequent peroxiredoxin-III, which is a primary line of defense against H2O2 in mitochondria.
- Subjects
THIOREDOXIN; THIOLS; PROTEINS; NECROSIS
- Publication
FEBS Letters, 2004, Vol 567, Issue 2/3, p189
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2004.04.055