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- Title
Automated hollow-fiber liquid-phase microextraction followed by liquid chromatography with mass spectrometry for the determination of benzodiazepine drugs in biological samples.
- Authors
Nazaripour, Ali; Yamini, Yadollah; Ebrahimpour, Behnam; Fasihi, Javad
- Abstract
In this study, two-phase hollow-fiber liquid-phase microextraction and three-phase hollowfiber liquid-phasemicroextraction based on two immiscible organic solvents were compared for extraction of oxazepam and Lorazepam. Separations were performed on a liquid chromatography with mass spectrometry instrument. Under optimal conditions, three-phase hollow-fiber liquid-phase microextraction based on two immiscible organic solvents has a better extraction efficiency. In a urine sample, for three-phase hollow fiber liquid-phase microextraction based on two immiscible organic solvents, the calibration curves were found to be linear in the range of 0.6-200 and 0.9-200 μg L-1 and the limits of detection were 0.2 and 0.3 μg L-1 for oxazepam and lorazepam, respectively. For two-phase hollow fiber liquidphase microextraction, the calibration curves were found to be linear in the range of 1-200 and 1.5-200 μg L-1 and the limits of detection were 0.3 and 0.5 μg L-1 for oxazepam and lorazepam, respectively. In a urine sample, for three-phase hollow-fiber-based liquid-phase microextraction based on two immiscible organic solvents, relative standard deviations in the range of 4.2-4.5% and preconcentration factors in the range of 70-180 were obtained for oxazepam and lorazepam, respectively. Also for the two-phase hollow-fiber liquid-phasemicroextraction, preconcentration factors in the range of 101-257 were obtained for oxazepam and lorazepam, respectively.
- Subjects
EXTRACTION (Chemistry); OXAZEPAM (Drug); LORAZEPAM; ORGANIC solvents; LIQUID chromatography; BENZODIAZEPINES
- Publication
Journal of Separation Science, 2016, Vol 39, Issue 13, p2595
- ISSN
1615-9306
- Publication type
Article
- DOI
10.1002/jssc.201600015