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- Title
The role of miRNA-377 as a tumor suppressor in lung cancer by negative regulation of genes belonging to ErbB signaling pathway.
- Authors
Hashemi, Saba; Yari, Naghmeh; Rahimi Jamnani, Fatemeh; Mahdian, Reza; Karimi, Morteza; Zeinali, Sirous; Rafiee, Mohammad Hesam; Azizi, Masoumeh
- Abstract
Background: The ErbB signaling pathway plays important role in the pathogenesis of lung cancer. We explored the role of miRNA-377 as a tumor suppressor in NSCLC through silencing of some genes in the ErbB pathway. Methods and Results: The targeting effect of miRNA-377 on EGFR, MAPK1, ABL2, and PAK2 was evaluated. The expression levels of these genes and miRNA-377 were surveyed in NSCLC and normal human tissues, Calu-6, and A549 cells. Real-time PCR was used to figure out whether miRNA-377 could decrease the target genes mRNAs in transfected lung cancer cell lines. The effects of miRNA-377 on apoptosis cell and proliferation were analyzed. We showed that miRNA-377 targets EGFR, MAPK1, and PAK2 mRNAs in in-silico and luciferase reporter assay. The expression of miRNA-377 was significantly downregulated in human NSCLC tissues, Calu-6 and A549 cells compared to their controls. We observed a negative correlation between EGFR, MAPK1, PAK2, and miRNA-377 expression in human NSCLC tissues. A significant reduction in EGFR, MAPK1, and PAK2 mRNA levels was detected, following miRNA-377 transfection in Calu-6 and A549 cells. The higher levels of miRNA-377 in Calu-6, and A549 cells induced apoptosis and reduced proliferation, significantly. Conclusions: All these data reveal that miRNA-377 functions as a tumor suppressor in NSCLC and may serve as a potential therapeutic target for the treatment of NSCLC.
- Subjects
TUMOR suppressor genes; LUNG cancer; GENETIC regulation; CELLULAR signal transduction; LUNG tumors; GENE silencing
- Publication
Molecular Biology Reports, 2022, Vol 49, Issue 1, p85
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-021-06844-6