We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Apolipoprotein A5 polymorphisms in Turkish population: association with serum lipid profile and risk of ischemic stroke.
- Authors
Can Demirdöğen, Birsen; Şahin, Esra; Türkanoğlu Özçelik, Aysun; Bek, Semai; Demirkaya, Şeref; Adali, Orhan
- Abstract
Atherosclerosis, a major cause of ischemic stroke, may be associated with variability of triglyceride (TG) levels. Apolipoprotein A5 ( APOA5) genetic polymorphisms are associated with altered TG levels. The objective of this study was to investigate the coding region polymorphisms S19W (rs3135506) and G185C (rs2075291) and the promoter region polymorphism −1131T>C (rs662799) of the APOA5 gene as risk factors for ischemic stroke in Turkish population. Study group consisted of 272 ischemic stroke patients and 123 controls. Genotypes were determined by real-time polymerase chain reaction (PCR) for S19W and PCR-restriction fragment length polymorphism analysis (PCR-RFLP) for −1131T>C and G185C. 19W allele frequency was 0.090 in stroke patients and 0.062 in controls ( P = 0.191). Minor allele frequencies of −1131T>C and G185C in patients were 0.106 and 0.004, respectively, and were nearly the same in controls. Total cholesterol and LDL-cholesterol levels were significantly higher for stroke patients having at least one 19W allele compared to non-carriers. A significant difference was also found for LDL-cholesterol levels of stroke patients; higher in −1131C allele carriers compared to wild type patients. There was a trend for higher frequency of ischemic stroke among −1131C allele carrier hypertensive, diabetic or obese subjects compared to non-carriers. However, APOA5 genotypes were not associated with the risk of ischemic stroke by logistic regression analysis. The present study demonstrated that carrying rare alleles of APOA5 S19W, −1131T>C and G185C alone do not constitute a risk for ischemic stroke in the studied Turkish subjects.
- Subjects
TURKEY; APOLIPOPROTEIN A; APOLIPOPROTEINS; BLOOD lipids; TRIGLYCERIDES
- Publication
Molecular Biology Reports, 2012, Vol 39, Issue 12, p10459
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-012-1926-z