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- Title
Regeneration of Transected Recurrent Laryngeal Nerve Using Hybrid-Transplantation of Skeletal Muscle-Derived Stem Cells and Bioabsorbable Scaffold.
- Authors
Kazuno, Akihito; Maki, Daisuke; Yamato, Ippei; Nakajima, Nobuyuki; Seta, Hiroya; Soeda, Shuichi; Ozawa, Soji; Uchiyama, Yoshiyasu; Tamaki, Tetsuro
- Abstract
Hybrid transplantation of skeletal muscle-derived multipotent stem cells (Sk-MSCs) and bioabsorbable polyglyconate (PGA) felt was studied as a novel regeneration therapy for the transected recurrent laryngeal nerve (RLN). Sk-MSCs were isolated from green fluorescence protein transgenic mice and then expanded and transplanted with PGA felt for the hybrid transplantation (HY group) into the RLN transected mouse model. Transplantation of culture medium (M group) and PGA + medium (PGA group) were examined as controls. After eight weeks, trans-oral video laryngoscopy demonstrated 80% recovery of spontaneous vocal-fold movement during breathing in the HY group, whereas the M and PGA groups showed wholly no recoveries. The Sk-MSCs showed active engraftment confined to the damaged RLN portion, representing favorable prevention of cell diffusion on PGA, with an enhanced expression of nerve growth factor mRNAs. Axonal re-connection in the HY group was confirmed by histological serial sections. Immunohistochemical analysis revealed the differentiation of Sk-MSCs into Schwann cells and perineurial/endoneurial cells and axonal growth supportive of perineurium/endoneurium. The number of axons recovered was over 86%. These results showed that the stem cell and cytokine delivery system using hybrid transplantation of Sk-MSCs/PGA-felt is a potentially practical and useful approach for the recovery of transected RLN.
- Subjects
LARYNGEAL nerve palsy; SKELETAL muscle; MULTIPOTENT stem cells; FLUORESCENCE; TRANSGENIC mice; LARYNGOSCOPY; THERAPEUTIC use of cytokines; TRANSPLANTATION of organs, tissues, etc.
- Publication
Journal of Clinical Medicine, 2018, Vol 7, Issue 9, p276
- ISSN
2077-0383
- Publication type
Article
- DOI
10.3390/jcm7090276