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- Title
The GRV2/RME-8 protein of Arabidopsis functions in the late endocytic pathway and is required for vacuolar membrane flow.
- Authors
Silady, Rebecca A.; Ehrhardt, David W.; Jackson, Karen; Faulkner, Christine; Oparka, Karl; Somerville, Chris R.
- Abstract
The gravitropism defective 2 ( grv2) mutants of Arabidopsis thaliana were previously characterized as exhibiting shoot agravitropism resulting from mutations in a homolog of the Caenorhabditis elegans RECEPTOR-MEDIATED ENDOCYTOSIS-8 ( RME-8) gene, which is required in C. elegans for endocytosis. A fluorescent protein fusion to the GRV2 protein localized to endosomes in transgenic plants, and vacuolar morphology was altered in grv2 mutants. A defect in vacuolar membrane dynamics provides a mechanistic explanation for the gravitropic defect, and may also account for the presence of an enlarged vacuole in early embryos, together with a nutrient requirement during seedling establishment. The GRV2-positive endosomes were sensitive to Wortmannin but not brefeldin A (BFA), consistent with GRV2 operating late in the endocytic pathway, prior to delivery of vesicles to the central vacuole. The specific enlargement of GRV2:YFP structures by Wortmannin, together with biochemical data showing that GRV2 co-fractionates with pre-vacuolar markers such as PEP12/SYP21, leads us to conclude that in plants GRV2/RME-8 functions in vesicle trafficking from the multivesicular body/pre-vacuolar compartment to the lytic vacuole.
- Subjects
ARABIDOPSIS thaliana; GEOTROPISM; CAENORHABDITIS elegans; TRANSGENIC plants; PLANT genetic engineering; PLANT cell culture; ENDOCYTOSIS
- Publication
Plant Journal, 2008, Vol 53, Issue 1, p29
- ISSN
0960-7412
- Publication type
Article
- DOI
10.1111/j.1365-313X.2007.03314.x