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- Title
Cysteine and serine proteases in gastric cancer.
- Authors
Plebani, Mario; Herszènyi, Làszlò; Cardin, Romilda; Roveroni, Giovanni; Carraro, Paolo; Paoli, Massimo D.; Rugge, Massimo; Grigioni, Walter F.; Nitti, Donato; Naccarato, Remo; Farinati, Fabio; Plebani, M; Herszènyi, L; Cardin, R; Roveroni, G; Carraro, P; Paoli, M D; Rugge, M; Grigioni, W F; Nitti, D
- Abstract
<bold>Background: </bold>Cysteine proteases (cathepsin B [CATB] and cathepsin L [CATL]), the serine protease urokinase-type plasminogen activator (UPA), and plasminogen activator inhibitor type-1 (PAI-1) are thought to play an important part in cancer invasion and metastasis. The aims of this study were to measure CATB, CATL, UPA, and PAI-1 in gastric cancer (GC) and normal mucosa distant from the tumor (NORM); to evaluate whether tissue levels are related to tumor stage, grade, or histotype; to assess their prognostic relevance; and to examine UPA and PAI-1 expression immunohistochemically.<bold>Methods: </bold>Gastric cancer and NORM samples were obtained from 25 patients with gastric cancer patients undergoing surgery (17 males, 8 females; mean age, 62 years; range, 31-84 years). Antigen concentrations were measured using the enzyme-linked immunosorbent assay method. Immunohistochemistry was performed using monoclonal UPA and PAI-1 antibodies.<bold>Results: </bold>Significantly higher antigen levels were found: (1) in GC vs. NORM (CATB, CATL, UPA, PAI-1) tissues; (2) in GC with versus without metastasis (CATB, CATL, UPA); (3) in poorly or moderately versus well differentiated GC; and (4) in diffuse versus intestinal-type GC (CATB, CATL). Urokinase-type plasminogen activator, PAI-1 and CATB levels had a significant prognostic impact. Cancer and stromal cells, showed immunoreactivity to anti-UPA and anti-PAI-1 antibodies.<bold>Conclusions: </bold>These results confirm the important role of CATB, CATL, UPA and PAI-1 in gastric cancer progression. Higher levels are detected in GC with metastases, poorer differentiation, and diffuse histotype, thus identifying patients with a worse prognosis.
- Publication
Cancer (0008543X), 1995, Vol 76, Issue 3, p367
- ISSN
0008-543X
- Publication type
journal article
- DOI
10.1002/1097-0142(19950801)76:3<367::AID-CNCR2820760304>3.0.CO;2-B