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- Title
Monomeric chemically modified allergens: immunologic and physicochemical characterization.
- Authors
Mistrello, G.; Brenna, O.; Roncarolo, D.; Zanoni, D.; Gentili, M.; Falagiani, P.
- Abstract
Allergenic extracts (<em>Der p, grass</em>, and <em>Parietaria</em>) or single allergens such as <em>Par j</em> I (the major allergen of <em>Parietaria</em>) and ovalbumin (OA), a food allergen widely used in animal models, were chemically modified by reaction with potassium cyanate (KCNO), which transforms the epsilon-amino group of the lysine of proteinaceous allergens into ureido groups. KCNO-modified (carbamylated) allergens have low allergenic potency, as demonstrated <em>in vitro</em> (RAST inhibition) and <em>in vivo</em> (passive cutaneous anaphylaxis). When used to immunize rabbits, carbamylated allergens still induce IgG antibodies able to cross-react with native allergens (immunoblotting experiments). An interesting feature distinguishing carbamylated allergens from other chemically modified allergens is the preservation of the native monomeric dimension as demonstrated by SDS-PAGE analysis. Results are discussed from the perspective of clinical application of carbamylated allergens.
- Subjects
ALLERGENS; POTASSIUM cyanide; PRIONS; IMMUNIZATION; IMMUNOGLOBULINS
- Publication
Allergy, 1996, Vol 51, Issue 1, p8
- ISSN
0105-4538
- Publication type
Article
- DOI
10.1111/j.1398-9995.1996.tb00003.x