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- Title
Proteomic analysis of ethanol-induced embryotoxicity in cultured post-implantation rat embryos.
- Authors
Makoto Usami; Katsuyoshi Mitsunaga; Tomohiko Irie; Atsuko Miyajima; Osamu Doi
- Abstract
Protein expression changes were examined in day 10.5 rat embryos cultured for 24 hr in the presence of ethanol by using two-dimensional electrophoresis and mass spectrometry. Exposure to ethanol resulted in quantitative changes in many embryonic protein spots (16 decreased and 28 increased) at in vitro embryotoxic concentrations (130 and 195 mM); most changes occurred in a concentration-dependent manner. For these protein spots, 17 proteins were identified, including protein disulfide isomerase A3, alpha-fetoprotein, phosphorylated cofilin-1, and serum albumin. From the gene ontology classification and pathway mapping of the identified proteins, it was found that ethanol affected several biological processes involving oxidative stress and retinoid metabolism.
- Subjects
PROTEOMICS; EMBRYOLOGY; EMBRYO implantation; ETHANOL; LABORATORY rats; PHOSPHORYLATION; ALPHA fetoproteins; PROTEIN disulfide isomerase; PHYSIOLOGY
- Publication
Journal of Toxicological Sciences, 2014, Vol 39, Issue 2, p285
- ISSN
0388-1350
- Publication type
Article
- DOI
10.2131/jts.39.285