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- Title
Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose).
- Authors
Wang, Tong; Coshic, Kush; Badiee, Mohsen; McDonald, Maranda R.; Aksimentiev, Aleksei; Pollack, Lois; Leung, Anthony K. L.
- Abstract
Poly(ADP-ribose) (PAR), a non-canonical nucleic acid, is essential for DNA/RNA metabolism and protein condensation, and its dysregulation is linked to cancer and neurodegeneration. However, key structural insights into PAR's functions remain largely uncharacterized, hindered by the challenges in synthesizing and characterizing PAR, which are attributed to its length heterogeneity. A central issue is how PAR, comprised solely of ADP-ribose units, attains specificity in its binding and condensing proteins based on chain length. Here, we integrate molecular dynamics simulations with small-angle X-ray scattering to analyze PAR structures. We identify diverse structural ensembles of PAR that fall into distinct subclasses and reveal distinct compaction of two different lengths of PAR upon the addition of small amounts of Mg2+ ions. Unlike PAR15, PAR22 forms ADP-ribose bundles via local intramolecular coil-to-globule transitions. Understanding these length-dependent structural changes could be central to deciphering the specific biological functions of PAR. This study reveals the 3D structures of poly(ADP-ribose) (PAR) using small-angle X-ray scattering and molecular dynamics simulations. It demonstrates how cation concentration and polymer length influence PAR's structure, providing insights into how a homopolymer can have specific functional roles.
- Subjects
SMALL-angle X-ray scattering; MOLECULAR dynamics; NUCLEIC acids; PROTEIN metabolism; CARRIER proteins
- Publication
Nature Communications, 2024, Vol 15, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-51972-9