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- Title
Characterization of Escherichia coli [NiFe]-Hydrogenase Distribution During Fermentative Growth at Different pHs.
- Authors
Trchounian, Karen; Pinske, Constanze; Sawers, R.; Trchounian, Armen
- Abstract
The contribution made by each of the three active [NiFe]-hydrogenases (Hyd) of Escherichia coli during fermentation of glucose or glycerol in peptone-based medium at different pHs was analysed. The activities of the hydrogen-oxidizing Hyd-1 and Hyd-2 enzymes showed a reciprocal dependence on the pH of the medium while Hyd-3, a key component of the hydrogen-evolving formate hydrogenlyase complex, was mainly active at pH 6.5. Our findings identify the conditions during fermentation of glucose or glycerol under which each [NiFe]-hydrogenase is optimally active and demonstrate a previously unrecognized dependence on Hyd-1 activity at low pH.
- Subjects
ESCHERICHIA coli; HYDROGENASE; GLUCOSE; GLYCERIN; PEPTONES; HYDROGEN
- Publication
Cell Biochemistry & Biophysics, 2012, Vol 62, Issue 3, p433
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-011-9325-y