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- Title
Inhibitory effects of N,N,N -trimethyl phytosphingosine-iodide on melanogenesis via ERK activation-mediated MITF degradation.
- Authors
Lee, Woo Jin; Bang, Seunghyun; Chung, Bo Young; Jung, Hyejung; Oh, Eok Soo; Chang, Sung Eun
- Abstract
N,N,N-trimethyl phytosphingosine-iodide (TMP) was recently developed as an antitumor agent. We examined the effects of TMP on melanogenesis and its related signaling pathways in normal human melanocytes. Our results showed that melanin is significantly reduced in a dose-dependent manner in both cells following liposomal TMP treatment. We also investigated changes in the phosphorylation of extracellular signal-regulated kinase (ERK), which is related to the degradation of microphthalmia-associated transcription factor (MITF). Our results indicated that liposomal TMP treatment leads to the phosphorylation of ERK, which reduces both MITF and tyrosinase protein levels. Treatment with PD98059, an ERK pathway-specific inhibitor, restored liposomal TMP-induced reductions in melanin, abrogated reductions in tyrosinase activity, and downregulated MITF and tyrosinase protein. In conclusion, these results suggest that the inhibitory effects of TMP on melanogenesis are due to MITF and tyrosinase downregulation via ERK activation. Liposomal TMP treatment decreases the expression of both MITF and tyrosinase proteins
- Subjects
MELANOGENESIS; EXTRACELLULAR signal-regulated kinases; MICROPHTHALMIA-associated transcription factor
- Publication
Bioscience, Biotechnology & Biochemistry, 2016, Vol 80, Issue 1, p121
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2015.1072459