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- Title
Sensitive detection of the c- KIT c. 1430G>T mutation by mutant-specific polymerase chain reaction in feline mast cell tumours.
- Authors
Takanosu, M.; Sato, M.; Kagawa, Y.
- Abstract
Here, we describe the establishment of mutant-specific polymerase chain reaction ( PCR) for detection of a c- KIT c. 1430G>T mutation in feline mast cell tumours. Several mutations in feline c- KIT have been identified, with the c. 1430G>T mutation accounting for a significant portion of feline mast cell tumour mutations. The c. 1430G>T mutation in c- KIT exon 9 was detected in 15.7% (11 of 70) of samples by mutant-specific PCR but in only 7.1% (5 of 70) by PCR-restriction fragment length polymorphism ( RFLP) in the genomic DNA isolated from 70 formalin-fixed paraffin-embedded sections or cells collected by fine needle aspiration. Mutant-specific PCR showed remarkably higher detection rate than did PCR-RFLP. DNA sequence analysis did not always yield identical results to those of mutant-specific PCR, suggesting heterogeneity of tumour cells. Mutant-specific PCR is a valid and efficient screening tool for detection of the c- KIT c. 1430G>T point mutation in feline mast cell tumours compared with PCR-RFLP and sequencing analysis.
- Subjects
GENETIC mutation; POLYMERASE chain reaction; MAST cell tumors; CAT diseases; GENETIC polymorphisms; DNA; FORMALDEHYDE
- Publication
Veterinary & Comparative Oncology, 2014, Vol 12, Issue 2, p138
- ISSN
1476-5810
- Publication type
Article
- DOI
10.1111/j.1476-5829.2012.00346.x