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- Title
Multiplexed Determination of Amino-Terminal Pro-B-Type Natriuretic Peptide and C-Reactive Protein Cardiac Biomarkers in Human Serum at a Disposable Electrochemical Magnetoimmunosensor.
- Authors
de Ávila, Berta Esteban‐Fernández; Escamilla‐Gómez, Vanessa; Lanzone, Valentina; Campuzano, Susana; Pedrero, María; Compagnone, Dario; Pingarrón, José M.
- Abstract
A rapid magnetoimmunosensor for the simultaneous determination of two cardiac biomarkers, amino-terminal pro-B-type natriuretic peptide (NT-proBNP) and C-reactive protein (CRP), in human serum is described. Specific capture antibodies were covalently immobilized onto carboxylic acid-modified magnetic beads. The quantification of NT-proBNP and CRP was performed by using indirect competitive and sandwich configurations, respectively, and horseradish peroxidase-labeled tracers. The use of dual screen-printed carbon electrodes allowed the achievement of simultaneous independent amperometric readout for each cardiac biomarker. The developed methodology showed very low limits of detection (0.47 ng mL−1). An international standard for CRP serum spiked with NT-proBNP was analyzed to evaluate the usefulness of the magnetoimmunosensor.
- Subjects
NATRIURETIC peptides; C-reactive protein; BIOMARKERS; SERUM; HORSERADISH peroxidase; CARBON electrodes; HEART
- Publication
Electroanalysis, 2014, Vol 26, Issue 2, p254
- ISSN
1040-0397
- Publication type
Article
- DOI
10.1002/elan.201300479