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- Title
Effect of Tags and Labels on the Performance of Enzyme-Amplified Electrochemical Genomagnetic Assays.
- Authors
González‐Álvarez, María José; Pérez‐Ruiz, Elena; Miranda‐Castro, Rebeca; de‐los‐Santos‐Álvarez, Noemí; Miranda‐Ordieres, Arturo J.; Lobo‐Castañón, María Jesús
- Abstract
A magnetic microsphere-based hybridization assay for the electrochemical detection of pathogen-specific DNA sequences is reported. Enzyme-linked sandwich hybridization assays with different tags (biotin, fluorescein or digoxigenin) and enzyme conjugates (streptavidin-alkaline phosphatase, streptavidin-peroxidase, antifluorescein-peroxidase or antidigoxigenin-peroxidase) are comparatively evaluated. Sensitivity and limit of detection depend on both the choice of the enzyme and the tag. The highest sensitivity and lowest limit of detection were obtained when antibody Fab fragments conjugated to peroxidase were used as reporter molecule. The digoxigenin system yields the lowest detection limit, mainly due to producing much lower background signals.
- Publication
Electroanalysis, 2013, Vol 25, Issue 1, p147
- ISSN
1040-0397
- Publication type
Article
- DOI
10.1002/elan.201200381