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- Title
Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC‐MS.
- Authors
Zhu, Ying; Clair, Geremy; Chrisler, William B.; Shen, Yufeng; Zhao, Rui; Shukla, Anil K.; Moore, Ronald J.; Misra, Ravi S.; Pryhuber, Gloria S.; Smith, Richard D.; Ansong, Charles; Kelly, Ryan T.
- Abstract
Abstract: We report on the quantitative proteomic analysis of single mammalian cells. Fluorescence‐activated cell sorting was employed to deposit cells into a newly developed nanodroplet sample processing chip, after which samples were analyzed by ultrasensitive nanoLC‐MS. An average of circa 670 protein groups were confidently identified from single HeLa cells, which is a far greater level of proteome coverage for single cells than has been previously reported. We demonstrate that the single‐cell proteomics platform can be used to differentiate cell types from enzyme‐dissociated human lung primary cells and identify specific protein markers for epithelial and mesenchymal cells.
- Subjects
PROTEOMICS; CELL-mediated cytotoxicity; CELL proliferation; CELL culture; CELL differentiation
- Publication
Angewandte Chemie, 2018, Vol 130, Issue 38, p12550
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201802843