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- Title
Identification of new resistance source for Sclerotinia stem rot in backcross population of B. juncea + S. alba allohexaploids: key to manage disease through host resistance.
- Authors
Kumari, Preetesh; Singh, Kaushal Pratap; Rai, Pramod Kumar
- Abstract
Sclerotinia sclerotiorum, which causes Sclerotinia stem rot, poses a significant threat to the global production of mustard and rapeseed crops. Cultivated Brassica germplasm does not contain a source of resistance to S. sclerotiorum. Rapeseed and mustard have been utilized to introduce the S. sclerotiorum resistance, nevertheless, using wild Brassicaceae members. In the current work, we developed a backcross population from four stable somatic hybrids of Sinapis alba and B. juncea to identify fertile lines that are resistant to stem rot disease. Each backcross line had extremely high male and female fertility and had successfully backcrossed with a B. juncea parent. In two different locations, a collection of 411 lines, including somatic hybrids, BC1F2-3, and BC2F1-2 lines, as well as tolerant and susceptible checks, were tested using two different virulent strains of the pathogen. The study's findings demonstrated that somatic hybrids and the BC1F2-3 lines were resistant to stem rot, whereas the BC2F1-2 lines displayed varied expressions of resistance to the disease, ranging from immune to highly susceptible. While the B. carinata remained tolerant in our investigation, the susceptible parent and checks were rated as highly susceptible. In addition, the in-vitro cotyledon assay for stem rot demonstrated that hybrids and the BC1F4 generation consistently responded with a resistance response, however, the BC2F3 generation responded differently, as it would under actual field conditions. It was discovered that the stem cortex and pith thickness are significant factors connected to the resistance response. To the best of our knowledge, this is the first report to identify immune and highly resistant B. juncea backcross lines with an alien genome in such a large number that they could easily hybridize with other B. juncea germplasms and use in resistance breeding programs for the Sclerotinia stem rot disease.
- Subjects
DISEASE resistance of plants; SOMATIC hybrids; BRASSICA juncea; SCLEROTINIA sclerotiorum; NATURAL immunity; RAPESEED
- Publication
Euphytica, 2023, Vol 219, Issue 7, p1
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-023-03208-y