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- Title
NGS-based genome wide association study helps to develop co-dominant marker for the physical map-based locus of PFRU controlling flowering in cultivated octoploid strawberry.
- Authors
Saiga, Sorachi; Tada, Maiko; Segawa, Tenta; Sugihara, Yu; Nishikawa, Minami; Makita, Natsu; Sakamoto, Minami; Tanaka, Kanako; Wada, Takuya; Takagi, Hiroki
- Abstract
Altering the flowering habit of strawberry (Fragaria × ananassa) from seasonal flowering to perpetual flowering (PF) can extend its production period and increase yields. Traditional linkage analysis with DNA markers revealed that the single dominant Perpetual Flowering Runnering (PFRU) locus controls the PF trait in strawberry. Additional DNA markers for PFRU were developed using the linkage map; however, they have only been applied to the limited cultivars showing polymorphisms at this locus. Furthermore, only dominant markers were previously available, making it difficult to distinguish PFRU homozygotes and heterozygotes. Here, we delimited the PFRU genomic region by performing a genome-wide association study with whole-genome sequencing data and a publicly available reference genome and mapped the previously reported DNA markers onto a physical map of F. × ananassa. Our study will facilitate the development of more DNA markers to accelerate breeding PF-type strawberry cultivars and further delimit the candidate region to isolate the causal gene for PFRU.
- Subjects
GENOME-wide association studies; LOCUS of control; CULTIVARS; STRAWBERRIES; DNA analysis; GENETIC markers; GENE mapping
- Publication
Euphytica, 2023, Vol 219, Issue 1, p1
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-022-03132-7