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- Title
One-step purification of xylanase from Melanocarpus albomyces and ethylene glycol as a novel soluble additive for enhancing its thermal stability.
- Authors
Gupta, Anshu; Roy, Ipsita; Khare, S.K.; Bisaria, V.S.; Gupta, M.N.
- Abstract
Hydrophobic interaction chromatography with Phenyl Sepharose 6 Fast Flow resulted in 7-fold purification of xylanaseactivity from Melanocarpus albomyces with over 60% recovery of theactivity. The purified preparation consisted of two major isoenzymes out of seven present in the organism. Ethyleneglycol, used as the eluent, enhanced the thermal stability of the xylanase activity from a half-life of 2.2 h at 55 °C to almost 40 h.
- Subjects
BIOLOGICAL nutrient removal; ETHYLENE glycol; XYLANASES; ISOENZYMES; AGAR; HYDROPHOBIC surfaces; GEL permeation chromatography
- Publication
Biotechnology Letters, 2002, Vol 24, Issue 23, p2005
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1021114831650