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- Title
Prejunctional Muscarinic Receptors Modulating Acetylcholine Release in Rabbit Detrusor Smooth Muscles.
- Authors
Inadome, Akito; Yoshida, Masaki; Takahashi, Wataru; Yono, Makoto; Seshita, Hiroshi; Miyamoto, Yutaka; Kawano, Takashi; Ueda, Shoichi
- Abstract
We investigated the presence and subtypes of functionally prejunctional receptors in cholinergic nerve endings of rabbit detrusor smooth muscle strips using high-performance liquid chromatography coupled with a microdialysis procedure. The effects of pretreatment with various drugs on acetylcholine (ACh) release and contractile responses induced by electrical field stimulation were evaluated. Although atropine (a muscarinic nonselective antagonist) and 4-DAMP (a muscarinic M[sub 3] antagonist) did not influence the ACh release, they markedly reduced the contractile responses. Pirenzepine (M[sub 1] antagonist) decreased ACh release and contractile responses. Methoctramine (M[sub 2] antagonist) increased the ACh release, but did not influence to the contractile responses. These results suggest that the muscarinic receptors in the rabbit detrusor smooth muscle are heterogeneous, prejunctional facilitatory (M[sub 1] receptors), and inhibitory (M[sub 2] receptors) for ACh release and postjunctional M[sub 3] receptors mediating contractile responses.
- Subjects
SMOOTH muscle diseases; LIQUID chromatography; ACETYLCHOLINE; CHROMATOGRAPHIC analysis; PIRENZEPINE; BENZODIAZEPINES
- Publication
Urologia Internationalis, 1998, Vol 61, Issue 3, p135
- ISSN
0042-1138
- Publication type
Article
- DOI
10.1159/000030309