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- Title
ALTERAÇÕES METABÓLICAS EM POMACEA MACULATA (MOLLUSCA) EXPERIMENTALMENTE INFECTADA COM ANGIOSTRONGYLUS CANTONENSIS (NEMATODA).
- Authors
dos Santos Castro, Lucas; Prenda da Silva, Jonathan de Souza; Rogel Carvalho Thiengo, Silvana Aparecida; Colli Montresor, Lângia; Pinheiro da Silva, Jairo
- Abstract
The mollusk Pomacea maculata belongs to the Gastropoda class and inhabits aquatic and terrestrial surfaces, has a herbivorous and non-specific feeding habit, being considered a major problem for small producers since it leads to the destruction of plantations. In addition, it acts as a host for the metastrongylid nematode Angiostrongylus cantonensis, a parasite of rodents and, occasionally, humans. The objective of this study was to evaluate glycemia by means of experimental infection, correlating the observed alterations with analyzes of glycogen and contents of organic acids related to oxidative metabolism during the pre-patent period of infection of P. maculata with A. cantonensis. Three groups of molluscs (I1, I2, I3) were used with 20 specimens each, infected with 10,000 L1 larvae per mollusc, each P. maculata having a shell length between 1.0 and 1.5 cm. Three other groups (C1, C2, C3) had the same number of uninfected animals and were maintained under the same conditions. Weekly, for three weeks after exposure to L1 larvae, a group of infected snails and another control group were dissected to collect soft tissue and hemolymph for analysis. Glycemia in infected molluscs was reduced in the first two weeks (50.91% and 65.84%, respectively), with a tendency to normalize at the end of the analysis period (+35.75%). The glycogen content in the head mass increased in the first two weeks of infection (+318.75% and +71.05%, respectively), with this content reduced by 51.11% in the third week. Glycogen concentration in the digestive gland varied significantly between infected snails compared to controls in the three analyzed weeks (+126.67%, +166.67% and +119.48% respectively), being always higher in infected snails. The concentration of pyruvic acid remained significantly lower in the infected molluscs in the first two weeks of infection (0.23mg/mL and 0.008mg/dL, respectively), with an increase of +75% in the concentration of pyruvate in the last week of infection. As for the concentrations of oxalic acid, these remained always lower in the infected molluscs in relation to the control (- 19.23%; -25.00%; -18.18%, respectively). Results are discussed indicating occurrence of synthesis de novo of glucose from non-glucose substrates and inhibition of carbohydrate degradation metabolism from tissue reserves.
- Subjects
ANGIOSTRONGYLUS cantonensis; CARBOHYDRATE metabolism; PYRUVIC acid; ORGANIC acids; TISSUE metabolism; OXALIC acid
- Publication
Revista Foco (Interdisciplinary Studies Journal), 2023, Vol 16, Issue 6, p1
- ISSN
1981-223X
- Publication type
Article
- DOI
10.54751/revistafoco.v16n6-155