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- Title
茯苓原生质体单核化及同核体杂交.
- Authors
李寿建; 董彩虹
- Abstract
Wolfiporia hoelen is an edible and medicinal fungus that has been cultivated in China for a long time and is cultivated currently on a large scale. However strain degeneration and decrease in yield caused by long-term asexual propagation affected the development of the industry. In order to solve the difficult breeding problem, distinguishing methods of homokaryotic strains and single-spore homokaryotic strain crossbreeding systems were constructed, and the mating system was disclosed in our previous studies. However, the difficulty of fruiting in some strains or attachment of some fruiting bodies to the media resulted in the difficulty of collecting basidiospores still remains unsettled. Therefore, the research on protoplast monokaryogenesis is important and meaningful. In the present study, the two strains, 775 and 776, with distant genetic relationship and different homokaryotic types, were used as experimental strains. Nuclear fluorescence staining revealed that more than 60% of protoplasts had nuclei, and the protoplast regeneration ratio was 7.6% and 11.0% respectively. The regenerative strains with growth stagnancy included both homokaryotic and heterokaryotic strains. The homokaryotic strains were collected with a ratio over 10%, and the homokaryotic strain 776 with different mating type was collected with a ratio of 5:3 with no partial separation (χ2 =0.5), and strain 775 with only one mating type was collected with a significant partial separation. By crossing of protoplast homokaryotic strains derived from protoplast and single spore, 25 and 50 hybrids were produced separately, which were confirmed by antagonism tests and rpb2 specific loci. This protoplast homokaryotic strain crossbreeding method built in W. hoelen was proved to be practicable.
- Subjects
CHINA; VEGETATIVE propagation; FRUITING bodies (Fungi); EDIBLE fungi; PROTOPLASTS; CROSSBREEDING
- Publication
Mycosystema, 2023, Vol 46, Issue 6, p1258
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.220239