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- Title
利用转录组信息揭示刺芹侧耳单核和双核菌丝中小RNA的表达差异.
- Authors
尚俊军; 汪滢; 龚明; 杨瑞恒; 唐利华; 万佳宁; 李焱; 茅文俊; 本田与一; 鲍大鹏; 李燕
- Abstract
The Illumina HiSeq 2500 high-throughput sequencing platform was used to sequence and analyze mycelial small RNA libraries of Pleurotus eryngii dikaryotic strain ‘Xinghan’ and its protoplast-derived monokaryotic strains ‘181’ and ‘183’. High-quality reads obtained by sequencing were compared with the Rfam database and the P. eryngii genome sequence. The results showed that 47.4% of the reads were derived from non-coding RNAs such as rRNAs, tRNAs, snRNAs, and snoRNAs, while 15.5% of the reads were derived from mRNA degradation. The remaining reads were compared with the miRBase database, and 946 small RNAs were found to match mature miRNAs of other species in the database. The expression of these small RNAs was further compared in monokaryotic and dikaryotic strains. Proportion of high-expression small RNAs was 19.4% greater in dikaryotic strain ‘Xinghan’ than that in monokaryotic strains. There were only 39 small RNAs up-regulated in ‘181’ and ‘183’, whereas 196 small RNAs were up-regulated in the dikaryotic strain.
- Subjects
NON-coding RNA; MICRORNA; NUCLEOTIDE sequencing; PLEUROTUS; MESSENGER RNA; TRANSFER RNA
- Publication
Acta Edulis Fungi, 2020, Vol 27, Issue 2, p17
- ISSN
1005-9873
- Publication type
Article
- DOI
10.16488/j.cnki.1005-9873.2020.02.003