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- Title
Predominant expression of growth hormone secretagogue receptor in the caudal lobe of chicken pituitary and parallel developmental increase in expression of pituitary GH and proventriculus ghrelin mRNA.
- Authors
Yamamoto, Ichiro; Yoshimura, Yukinori; Tsukada, Akira; Kansaku, Norio; Tsushima, Nobumichi; Tanaka, Minoru
- Abstract
To examine the involvement of ghrelin in growth hormone (GH) synthesis in the chicken pituitary, the regional distribution of GH secretagogue receptor (GHS-R)/ghrelin receptor was investigated. Quantitative real-time polymerase chain reaction (Q-PCR) analysis revealed that the expression levels of GHS-R and GH mRNA in the caudal lobe were about fourfold and sevenfold higher in the cephalic lobe of 7 day-old chickens, respectively. Immunohistochemical analysis showed that GHS-R immunoreactivity was more abundant in the caudal lobe than in the cephalic lobe, as was the case for GH immunoreactivity. By Q-PCR, parallel increases were observed in the expression levels of ghrelin mRNA in the proventriculus and GH mRNA in the pituitary from embryonic day 17 to day 7 after hatching, whereas no significant change was found in the expression levels of GHS-R mRNA in the pituitary during this period. These results suggest that proventriculus-derived ghrelin may participate in pituitary GH synthesis by acting on its receptor during late embryonic development and the early post-hatching period in chickens.
- Subjects
SOMATOTROPIN; POLYMERASE chain reaction; CHICKENS; GHRELIN; PITUITARY hormones
- Publication
Animal Science Journal, 2008, Vol 79, Issue 1, p116
- ISSN
1344-3941
- Publication type
Article
- DOI
10.1111/j.1740-0929.2007.00506.x