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- Title
四君子汤对溃疡性结肠炎小鼠模型 occludin 和 claudin-1 表达的影响.
- Authors
黄晓燕; 郑超伟; 罗莉川; 赵一娜; 王萌; 刘熙荣; 李生发
- Abstract
Objective: To explore the effect of Sijunzi decoction on occludin and claudin-1 expression in ulcerative colitis(UC) mice model. Methods: The UC mice model was induced by dextran sulfate sodium (DSS). The experiment was divided into five groups: normal group, model group, low-dose treatment group, middle-dose treatment group, high-dose treatment group and western medicine group. The treatment lasted for 7 days. The morphological and histopathological changes of intestinal mucosa in mice were observed. The mRNA and protein expressions of occludin and claudin-1 were detected by RT-PCR and Western blot. Results: Compared with the model group, the diet, body weight, spirit, activity and bloody purulent stool of the mice in the low, middle and high dose Sijunzi decoction treatment group and Western medicine group were improved, and the pathological manifestations such as mucosal defect, crypt destruction and inflammatory cell infiltration were alleviated. Compared with the model group, the protein and mRNA expressions of occludin and claudin-1 in the high-dose treatment group increased (P<0.05), and the protein and mRNA expressions of occludin and claudin-1 in the low-dose and middle-dose treatment groups also increased in varying degrees. Compared with the western medicine group, there was no significant difference in the protein and mRNA expressions of occludin and claudin-1 in the low, middle and high dose groups (P>0.05). Conclusion: Sijunzi Decoction can improve the symptoms of bloody purulent stool, relieve the damage of intestinal mucosa, up-regulate the expression of occludin and claudin-1, and has therapeutic effect on UC mice.
- Subjects
ULCERATIVE colitis; DEXTRAN sulfate; INTESTINAL mucosa; PROTEIN expression; SODIUM sulfate
- Publication
Progress in Modern Biomedicine, 2019, Vol 19, Issue 5, p829
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2019.05.007