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- Title
In vivo imaging of CREB phosphorylation in awake-mouse brain.
- Authors
Ishimoto, Tetsuya; Mano, Hiroki; Mori, Hisashi
- Abstract
The cyclic adenosine monophosphate response element binding protein (CREB) is a phosphorylation-dependent transcription factor that plays important roles in memory consolidation and several neuropsychological disorders. Although analyzing the spatiotemporal pattern of CREB phosphorylation is required for elucidating the mechanism of memory consolidation, imaging of phosphorylation of a particular protein in the brain of live animals is impossible at present. Here, we developed a method for visualizing the CREB phosphorylation in the cerebral cortex of an awake mouse using a split luciferase technique. Using this technique, we demonstrated the correlation between the change in CREB phosphorylation at a particular region in the brain and behavioral consequences induced by the administration of reserpine, a psychotropic agent.
- Subjects
CYCLIC adenylic acid; CARRIER proteins; PHOSPHORYLATION; CEREBRAL cortex; LUCIFERASES; RESERPINE
- Publication
Scientific Reports, 2015, p9757
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep09757