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- Title
The auxin responsive gene Pp-C61 is up-regulated inPinus pinaster roots following inoculation with ectomycorrhizal fungi.
- Authors
REDDY, S. M.; PANDEY, A. K.; MELAYAH, D.; MARMEISSE, R.; GAY, G.
- Abstract
An indoleacetic acid (IAA) up-regulated cDNA, referred to as Pp-C61, was isolated by differential screening of a cDNA library constructed from auxin-treated roots of Pinus pinaster. The Pp-C61 cDNA contains a 1068 bp open reading frame which encodes a polypeptide sharing no homology with known proteins. The N-terminal protein region contains a hydrophobic signal peptide suggesting that Pp-C61 is an extracellular protein. Pp-C61 is present as a single copy in the P. pinaster genome and homologous genes were detected in other gymnosperm and angiosperm trees. Pp-C61 was transcriptionally regulated by auxin with a maximal mRNA accumulation 6 h after treatment. PpC61 was up-regulated by IAA concentrations as low as 10[sup -10]M. Up-regulation was found to be a primary (direct) response to auxin. Ethylene and abscisic acid, but not querce fin were able to promote Pp-C61 mRNA accumulation. As a putative extracellular protein, Pp-C61 was postulated to play a role in ectomycorrhiza formation. Pp-C61 was upregulated in P. pinaster roots following inoculation with ectomycorrhizal fungi. Transcript accumulation was the highest (5.5-fold over control) in the presence of an IAAoverproducing mutant of Hebeloma cylindrosporum; it was less in the presence of wild-type strains of H. cylindrosporum and Rhizopogon roseolus. Pp-C61 transcript also accumulated upon inoculation with a non-mycorrhizal mutant of H. cylindrosporum. These results suggest that Pp-C61 up-regulation corresponds to a defence reaction in response to fungal colonization.
- Subjects
CLUSTER pine; ECTOMYCORRHIZAL fungi; AUXIN
- Publication
Plant, Cell & Environment, 2003, Vol 26, Issue 5, p681
- ISSN
0140-7791
- Publication type
Article
- DOI
10.1046/j.1365-3040.2003.01003.x