We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Kinetics of butyrate metabolism in the normal colon and in ulcerative colitis: the effects of substrate concentration and carnitine on the β-oxidation pathway.
- Authors
De Preter, V.; Geboes, K. P.; Bulteel, V.; Vandermeulen, G.; Suenaert, P.; Rutgeerts, P.; Verbeke, K.
- Abstract
Aliment Pharmacol Ther 2011; 34: 526-532 Summary Background Butyrate, a colonic metabolite of carbohydrates, is considered as the major energy source for the colonic mucosa. An impaired butyrate metabolism has been reported in ulcerative colitis (UC), however, the cause still remains unknown. Aim In the present study, we investigated whether higher butyrate concentrations could normalise the oxidation rate in UC. Furthermore, it was investigated whether carnitine could enhance the butyrate oxidation. Methods Mucosal biopsies from a total of 26 UC patients and 25 controls were incubated with 14C-labelled Na-butyrate and the produced 14CO2 was measured. First, the rate of oxidative metabolism was compared at three different concentrations of Na-butyrate (0.05 m m, 1 m m and 10 m m). Then, incubations of biopsies were performed with carnitine alone or combined with ATP. Results Overall, butyrate oxidation in UC was significantly lower than that in controls. The maximum rate of butyrate oxidation was achieved in UC and control subjects from 1 m m onwards. Increasing the butyrate concentration to a level to be present in the colonic lumen, i.e. 10 m m, did not increase the rate of butyrate oxidation in UC to the rate observed in controls. Addition of carnitine alone or combined with ATP caused no effects. Conclusions Saturation of butyrate kinetics was achieved from 1 m m in UC and control subjects. The rate of butyrate metabolism was significantly impaired in active ulcerative colitis. The addition of compounds interfering with the β-oxidation pathway had no effect on the butyrate metabolism in UC.
- Subjects
ULCERATIVE colitis; CARNITINE; BUTYRIC acid; KREBS cycle; MUCOUS membranes
- Publication
Alimentary Pharmacology & Therapeutics, 2011, Vol 34, Issue 5, p526
- ISSN
0269-2813
- Publication type
Article
- DOI
10.1111/j.1365-2036.2011.04757.x