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- Title
Morphological and ultrastructural changes in fungal agents after LASER application.
- Authors
Fernandes, Fernanda A. V.; Arruda, Denise C.; Santana, Gilglécia N. P.; Longo, Larissa V. G.; Cesar, Maria C. M.; da Matta, Daniel A.; Borba, Orion S. M.; Monteiro, Ruan C.; de Moura Bello, Marina; de Moura, Luís H. B.; Neto, Domingos J.; Yarak, Samira; de Camargo, Zoilo P.; Gompertz, Olga F.; Ogawa, Marília M.
- Abstract
Background: Onychomycosis is a fungal nail infection of difficult treatment due to the fungal survival capacity and reduced number of effective therapies. The present study aimed to isolate fungal agents that cause onychomycosis in immunocompetent patients and evaluate how LASER treatments affect the growth and ultrastructure of isolates. Methods: In total, 21 patients with positive direct microscopic examination (DME) for onychomycosis had nail samples collected for cultivation and phenotypic identification of microorganisms. From these patients, 12 underwent LASER treatment, divided in Group 1 (n = 5) treated with Nd: YAG 1,064 nm, and Group 2 (n = 7) treated with Nd: YAG 1,064 nm + Er: YAG 2,940 nm + topical isoconazole. Transmission Electron Microscopy (TEM) was performed to evaluate ultrastructural changes after treatment. Results: DME, cultivation, and phenotypic identification showed that the most identified fungus was Trichophyton rubrum spp. After LASER therapy, sample cultivation showed alterations in the fungal morphology with reduction of hyphae, conidia, and reproductive structures. Alterations in fungal cell wall structure, cytoplasm density, and organelles were observed by TEM. Conclusion: LASER irradiation causes changes in the fungal cells, especially in the number of hyphae and the presence of conidia. In addition, it affects fungal growth and reproduction capacity, which interferes with their infection ability and virulence.
- Subjects
FUNGAL cell walls; FUNGAL morphology; CHANGE agents; NAIL diseases; TRANSMISSION electron microscopy
- Publication
International Journal of Dermatology, 2024, Vol 63, Issue 2, p217
- ISSN
0011-9059
- Publication type
Article
- DOI
10.1111/ijd.16919