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- Title
The retinoblastoma protein interacts with Bag-1 in human colonic adenoma and carcinoma derived cell lines.
- Authors
Nathalie J. Arhel; Graham Packham; Paul A. Townsend; Tracey J. Collard; Akram M. H-Zadeh; Adam Sharp; Ramsey I. Cutress; Karim Malik; Angela Hague; Chris Paraskeva; Ann C. Williams
- Abstract
Although the retinoblastoma susceptibility gene RB1 is inactivated in a wide range of human tumours, overexpression in colonic carcinomas has been linked to the antiapoptotic function of the protein. In the current study we show that the Retinoblastoma susceptibility protein (Rb) protein interacts with Bag-1, an apoptotic regulator, in human colonic adenoma- and carcinoma-derived cell lines. Coimmunoprecipitation demonstrated that endogenous Rb and Bag-1 interact in both adenoma- and carcinoma-derived cell lines. The specificity of the interaction was demonstrated by expression of human Papillomavirus E7 oncoprotein, an inhibitor of Rb protein interactions, which disrupted the Rb/Bag-1 complex. We report that Bag-1 is predominantly localised in the nucleus of colorectal adenoma- and carcinoma-derived epithelial cells. Disruption of the Rb/Bag-1 complex through expression of E7 changes the subcellular distribution of Bag-1, decreasing nuclear localised Bag-1. Our work establishes that the Rb protein interacts with the Bag-1 apoptotic regulator protein, and introduces a novel function for Rb, involving modulation of the subcellular localisation of Bag-1 in human colonic epithelial cells. © 2003 Wiley-Liss, Inc.
- Subjects
RETINOBLASTOMA; CELL lines; CANCER cells; DISEASE susceptibility; ADENOMA; PROTEIN-protein interactions; PAPILLOMAVIRUSES; EPITHELIAL cells; GENETICS
- Publication
International Journal of Cancer, 2003, Vol 106, Issue 3, p364
- ISSN
0020-7136
- Publication type
Article
- DOI
10.1002/ijc.11257