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- Title
Development of a Monoclonal Antibody to Detect αs1-casein in the Milk of Healthy and Mastitis-Affected Goats.
- Authors
Ming PANG; Xiong GUAN; Chen-Xiang ZUO; Ming-Jie LIU; Saad REHMAN; Qin-Lei FAN; Ping LU; De-Kun CHEN; Wen-Tao MA
- Abstract
This study aimed to evaluate the expression level of caseins during mastitis of goats. Whole goat caseins were used as primary antigens for mouse immunization. A monoclonal antibody (mAb) named 5B with high specificity to goat αs1-casein was developed. Further results showed that mAb 5B can successfully be applied to western blot and ELISA. In addition, immunofluorescence analysis using this mAb showed increased milk αs1-casein level in mastitis-affected goats. In conclusion, this study has established an effective tool to evaluate the expression level of αs1-casein during mastitis development.
- Subjects
MONOCLONAL antibodies; MASTITIS; GOATS; GOAT milk; CASEINS; IMMUNOFLUORESCENCE; MILK; WESTERN immunoblotting
- Publication
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2020, Vol 26, Issue 1, p71
- ISSN
1300-6045
- Publication type
Article
- DOI
10.9775/kvfd.2019.22287