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- Title
Development and Evaluation of an ELISA System for Detection of PEDV-Specific IgA Antibodies in Colostrum.
- Authors
Ren-Feng LI; Xiang-Qin TIAN; Yan YU; Jin-Qing JIANG; San-Hu WANG; Zi-Liang WANG; Jin-You MA; Li-Rong WANG; Shou-Ping ZHANG; Yan-Yan YANG
- Abstract
Porcine epidemic diarrhea virus (PEDV) is a devastating swine infectious disease. Development of high-performance methods to diagnose and evaluate viral immune status remains very important to control PEDV. Here, a recombinant S1 protein-based indirect enzyme-linked immunosorbent assay (rpS1-iELISA) was developed to monitor IgA antibody in the colostrum. Optimized cut-off value of the rpS1-iELISA was determined as 0.448. Results yielded a sensitivity of 96.87% and a specificity of 100.0%. Repeatability tests indicated that the coefficients of variation of the colostrum samples within and between runs were both less than 8%. Test results of 523 field colostrum samples showed that the rpS1-iELISA had excellent agreement with immunofluorescence assay (kappa = 0.958) and better test performance than a commercial ELISA kit. This test will aid in future diagnostics and assessment of the protective levels of mucosal immune response against PEDV by measuring IgA levels in the colostrum.
- Subjects
PORCINE epidemic diarrhea virus; SWINE; ENZYME-linked immunosorbent assay; COLOSTRUM; IMMUNOGLOBULINS; ANIMAL health
- Publication
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2018, Vol 24, Issue 5, p717
- ISSN
1300-6045
- Publication type
Article
- DOI
10.9775/kvfd.2018.19776