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- Title
Laser-Scanning Microscope Imaging Method Based on Computer Graphics Processing Technology.
- Authors
Chao Jiang
- Abstract
Laser scanning microscopy is an effective technical means for studying microstructures and large scientific instruments, and is widely used in biological and industrial testing. The system uses precise pinhole filtering to detect only the focal plane position, minimize stray light in the unfocused plane, and has high imaging resolution and signal-to-noise ratio. When a laser beam is used to excite a sample, only one laser convergence point in the sample is excited. To form a complete image, an XY scanning galvanometer was introduced into the laser confocal scanning microscope to meet the requirements of a point-to-point progressive scan focal plane signal and to form a final complete confocal image. The Z-axis range is adjusted using a laser confocal scanning microscope, and continuous movement of the Z-axis can obtain continuous optical slice images of different layers of the sample. A stereoscopic image of an object is observed using a microscope, and a high-resolution stereoscopic image is used to achieve precise fine operation. In addition, accurate binocular microscope image measurements prior to precision machining ensure smooth subsequent image processing. Aiming at the problem that the stereo image has different binocular clarity at the same position, this paper proposes a laser-scanning microscope imaging method based on computer graphics processing technology. The method uses image reconstruction technology to restore the image to the ideal image, and uses the image filtering and image enhancement technology obtained under the microscope to study the image processing of the laser scanning microscope, which creates conditions and basis for in-depth microscopic imaging and medical analysis.
- Subjects
CONFOCAL microscopy; COMPUTER graphics; IMAGE processing; SCANNING electron microscopy; HIGH resolution imaging; SIGNAL-to-noise ratio
- Publication
Acta Microscopica, 2019, Vol 28, Issue 6, p1772
- ISSN
0798-4545
- Publication type
Article