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- Title
Dual functions of Arabidopsis sulfiredoxin: Acting as a redox-dependent sulfinic acid reductase and as a redox-independent nuclease enzyme
- Authors
Chi, Yong Hun; Kim, Sun Young; Jung, In Jung; Shin, Mi Rim; Jung, Young Jun; Park, Jin Ho; Lee, Eun Seon; Maibam, Punyakishore; Kim, Kang-San; Park, Joung Hun; Kim, Min Ji; Hwang, Gwang Yong; Lee, Sang Yeol
- Abstract
Abstract: Based on the fact that the amino acid sequence of sulfiredoxin (Srx), already known as a redox-dependent sulfinic acid reductase, showed a high sequence homology with that of ParB, a nuclease enzyme, we examined the nucleic acid binding and hydrolyzing activity of the recombinant Srx in Arabidopsis (AtSrx). We found that AtSrx functions as a nuclease enzyme that can use single-stranded and double-stranded DNAs as substrates. The nuclease activity was enhanced by divalent cations. Particularly, by point-mutating the active site of sulfinate reductase, Cys (72) to Ser (AtSrx-C72S), we demonstrate that the active site of the reductase function of AtSrx is not involved in its nuclease function.
- Subjects
NUCLEASES; ARABIDOPSIS; SULFIREDOXIN; OXIDATION-reduction reaction; SULFINIC acids; AMINO acid sequence; ENZYMATIC analysis
- Publication
FEBS Letters, 2012, Vol 586, Issue 19, p3493
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.08.002