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- Title
17beta-estradiol induces both up-regulation and processing of cyclin E in a calpain-dependent manner in MCF-7 breast cancer cells
- Authors
Hou, Jianmei; Wang, Xudong; Li, Yang; Liu, Xiaohong; Wang, Zhuting; An, Jing; Yang, Li; He, Yan
- Abstract
Abstract: In the current study, we investigated whether 17beta-estradiol (E2) induces cyclin E expression and triggers cyclin E processing via calpain in MCF-7 breast cancer cells. We found that E2 induced increased expression of cyclin E in a slow and persistent manner, and a rapid yet sustained processing of cyclin E. In addition, estrogenic ethanol was able to stimulate cyclin E truncation. Calpeptin or ALLN greatly suppressed the E2-triggered cyclin E processing and its expression, suggesting a calpain-mediated action for E2. Finally, the E2-induced effects could also be significantly suppressed by BAPTA or U0126, indicating involvement of calcium/ERK signaling. Taken together, these results show that estrogen may contribute to both up-regulation and proteolysis of cyclin E through calpain in MCF-7 cells.
- Subjects
ESTRADIOL; CYCLIN E; GENETIC regulation; CALPAIN; BREAST cancer; CANCER cells; CELL lines
- Publication
FEBS Letters, 2012, Vol 586, Issue 6, p892
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.02.018