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- Title
Induced recovery of defective membrane expression of a CC chemokine receptor 5 mutant by phytohemagglutinin.
- Authors
Qi, Q. -Y.; Wang, F.; Zhang, H. -T.; Wang, J. -C.; Xiao, H. -P; Wang, M. -H.; Han, Y. -F.; Zhang, R. -M.; Tao, S. -H.; Luo, Z. W.
- Abstract
CC chemokine receptor 5 (CCR5) is a member of the G-protein-coupled receptor superfamily. It plays an important role in macrophage tropic human immunodeficiency virus-1 entry and in some inflammatory reactions. CCR5-893(–) is a single-nucleotide deletion that results in complete truncation of the C tail of the gene product. We detected CCR5-893(–) in a sample of patients infected with non-tuberculosis mycobacteria and found that it was maintained heterozygously with a frequency of 2%. There is no association between this mutation and any immunodeficiency. Membrane expression of CCR5-893(–) was substantially reduced compared to the wild type, but this defective surface presentation recovered greatly recovered in the presence of 2 mg l-1 phytohemagglutinin (PHA). However, PHA inducement did not affect the total intracellular expression of CCR5-893(–) or wild-type CCR5. Thus we suggest there exist some PHA-induced factor(s) that could mediate the presentation of truncated CCR5.
- Subjects
CHEMOKINES; CYTOKINES; IMMUNODEFICIENCY; PHYTOHEMAGGLUTININS; MUCOPROTEINS
- Publication
Cellular & Molecular Life Sciences, 2003, Vol 60, Issue 11, p2492
- ISSN
1420-682X
- Publication type
Article
- DOI
10.1007/s00018-003-3295-1