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- Title
Effect of ProRoot MTA, Portland cement, and amalgam on the expression of fibronectin, collagen I, and TGFβ by human periodontal ligament fibroblasts in vitro.
- Authors
Fayazi, Sara; Ostad, Seyed Nasser; Razmi, Hasan
- Abstract
Context: Today many materials have been introduced for root-end filling materials. One of them is mineral trioxide aggregate (MTA) that is mentioned as a gold standard. Aims: The purpose of this in vitro study was to evaluate the reaction of human periodontal ligament fibroblasts to the root-end filling materials, such as ProRoot MTA, Portland cement, and amalgam. Settings and Design: Eight impacted teeth were extracted in aseptic condition. The tissues around the roots were usedto obtain fibroblast cells. After cell proliferation, they were cultured in the chamber slides and the extracts of the materials were added to the wells. Materials andMethods: Immunocytochemical method for measuring the expression of Fibronectin, collagen I and transforming growth factor beta (TGFβ) was performed by Olysia Bioreport Imaging Software. Statistical Analysis Used: The results were analyzed by SPSS 13.0 and Tukey post hoc test with P<0.05 as the limit of significance. Results: Collagen expression in MTA specimens was higher than the other groups in 24 h significantly. After 48 h, the Portland cement group showed the most expression of collagen significantly and after 1 week, Portland cement and MTA groups had the most expression of collagen but there was no significant difference between these 2 groups. After 1 week, the Portland cement group demonstrated a higher amount of TGFβ and fibronectin. Conclusions: The results suggest that Portland cement can be used as a less expensive root filling material with low toxicity. It has better effects than amalgam on the fibroblasts.
- Subjects
PORTLAND cement; DENTAL amalgams; FIBRONECTINS; PERIODONTAL disease treatment; ENDODONTICS; DENTAL fillings; TRANSFORMING growth factors-beta; EQUIPMENT &; supplies
- Publication
Indian Journal of Dental Research, 2011, Vol 22, Issue 2, p190
- ISSN
0970-9290
- Publication type
Article
- DOI
10.4103/0970-9290.84278