We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Associations of MTHFR and PAI-1 4G/5G Polymorphisms with Unexplained Recurrent Pregnancy Loss.
- Authors
Dao Anh Thi Le; Van Thanh Ta; Anh Duy Nguyen
- Abstract
Background and Objectives: Unexplained recurrent pregnancy loss (RPL) has been a challenging issue in diagnosis. Methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms (677C > T, 1298A > C) and plasminogen activator inhibitor type 1 (PAI-1) 4G/5G gene polymorphism have been proposed as risk factors of unexplained RPL. This study aimed to identify relationships between MTHFR 677C > T, 1298A > C gene polymorphisms and PAI-1 4G/5G gene polymorphism on unexplained RPL. Materials and Methods: A case-control study was performed on two groups: pregnant women with unexplained RPL (i.e., two or more pregnancy losses) as the case group, and those with at least one or more live births and no history of RPL as the control group. All pregnant women were assessed for the presence of MTHFR gene polymorphisms and PAI-1 4G/5G polymorphism by using the amplification refractory mutation system polymerase chain reaction (ARMS-PCR) technique. Associations between MTHFR 677C > T, 1298A > C gene polymorphisms, and PAI-1 4G/5G gene polymorphism with unexplained RPL were evaluated. Results: There were 73 pregnant women (43 cases and 30 controls) recruited. The rates of MTHFR 677C > T among cases were: TT allele 32.6%, CT allele 34.8%, and CC allele 32.6%, while the frequencies of these alleles were: TT alleles 10%, CT allele 36.7%, and CC allele 53.3% (p = 0.049). MTHFR 677C > T gene polymorphism was associated with an increased risk of unexplained RPL (for TT allele vs. CT + CC allele: OR = 3.25, 95%CI: 1.02-10.35, p = 0.028; for T vs. C: OR = 2.53, 95%CI: 1.25-5.11, p = 0.09). MTHFR 1298A > C gene polymorphism and PAI-1 4G/5G gene polymorphism were not related to unexplained RPL. Conclusion: MTHFR 677C > T gene polymorphism was related to unexplained RPL in Vietnamese pregnant women. Further longitudinal studies should be performed to examine this relationship.
- Subjects
GENETIC mutation; CONFIDENCE intervals; RECURRENT miscarriage; GENETIC polymorphisms; CASE-control method; PREGNANT women; ALLELES; PROTHROMBIN; RISK assessment; DESCRIPTIVE statistics; BLOOD coagulation factors; POLYMERASE chain reaction; ODDS ratio; WOMEN'S health; DISEASE risk factors
- Publication
International Medical Journal, 2022, Vol 29, Issue 1, p20
- ISSN
1341-2051
- Publication type
Article