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- Title
Utility of Plasma Microbial Cell-Free DNA Whole-Genome Sequencing for Diagnosis of Invasive Aspergillosis in Patients With Hematologic Malignancy or COVID-19.
- Authors
Lee, Ki Hyun; Won, Dongju; Kim, Jinnam; Lee, Jung Ah; Kim, Chang Hyup; Kim, Jung Ho; Jeong, Su Jin; Ku, Nam Su; Choi, Jun Yong; Yeom, Joon-Sup; Cho, Hyunsoo; Chung, Haerim; Cheong, June-Won; Lee, Seung-Tae; Jang, Ji Eun; Shin, Saeam; Ahn, Jin Young
- Abstract
Background We evaluated the clinical accuracy and utility of whole-genome sequencing (WGS) of plasma microbial cell-free DNA (cfDNA) as a novel noninvasive method in diagnosing invasive aspergillosis (IA) in patients with hematologic malignancy (HM) or coronavirus disease 2019 (COVID-19). Methods Adults with HM or COVID-19 and suspected IA were recruited. IA cases were retrospectively diagnosed according to EORTC/MSG definitions and ECMM/ISHAM criteria for HM and COVID-19 patients, respectively. The results of cfDNA WGS were compared with the conventional diagnosis. Results Microbial cfDNA WGS was performed 53 times from 41 participants (19 from HM, 16 from COVID-19, and 7 from the control group). In participants with HM, Aspergillus cfDNA was detected in 100% of proven IA and 91.7% of probable IA cases. In participants with COVID-19, 50.0% of probable IA were positive for Aspergillus in cfDNA WGS. Concordance between Aspergillus cfDNA detection and proven/probable IA conventional diagnosis was significantly higher in participants with HM than in those with COVID-19. IA diagnosed using EORTC/MGS definitions showed significantly high concordance between Aspergillus cfDNA detection and proven/probable IA. Conclusions Aspergillus cfDNA detection strongly correlated with proven/probable IA diagnosed using EORTC/MSG definitions and could be used as an additional diagnostic tool for IA.
- Subjects
CELL-free DNA; NUCLEOTIDE sequencing; HEMATOLOGIC malignancies; DNA sequencing; ASPERGILLOSIS; PULMONARY aspergillosis; CORONAVIRUS diseases
- Publication
Journal of Infectious Diseases, 2023, Vol 228, Issue 4, p444
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1093/infdis/jiad213