We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Transmission Dynamics and Microevolution of Neisseria meningitidis During Carriage and Invasive Disease in High School Students in Georgia and Maryland, 2006-2007.
- Authors
Mustapha, Mustapha M; Marsh, Jane W; Shutt, Kathleen A; Schlackman, Jessica; Ezeonwuka, Chinelo; Farley, Monica M; Stephens, David S; Wang, Xin; Tyne, Daria Van; Harrison, Lee H; Van Tyne, Daria
- Abstract
<bold>Background: </bold>The mechanisms by which Neisseria meningitidis cause persistent human carriage and transition from carriage to invasive disease have not been fully elucidated.<bold>Methods: </bold>Georgia and Maryland high school students were sampled for pharyngeal carriage of N. meningitidis during the 2006-2007 school year. A total of 321 isolates from 188 carriers and all 67 invasive disease isolates collected during the same time and from the same geographic region underwent whole-genome sequencing. Core-genome multilocus sequence typing was used to compare allelic profiles, and direct read mapping was used to study strain evolution.<bold>Results: </bold>Among 188 N. meningitidis culture-positive students, 98 (52.1%) were N. meningitidis culture positive at 2 or 3 samplings. Most students who were positive at >1 sampling (98%) had persistence of a single strain. More than a third of students carried isolates that were highly genetically related to isolates from other students in the same school, and occasional transmission within the same county was also evident. The major pilin subunit gene, pilE, was the most variable gene, and no carrier had identical pilE sequences at different time points.<bold>Conclusion: </bold>We found strong evidence of local meningococcal transmission at both the school and county levels. Allelic variation within genes encoding bacterial surface structures, particularly pilE, was common.
- Subjects
MARYLAND; GEORGIA; MENINGOCOCCAL infections; NEISSERIA meningitidis; HIGH school students; MICROEVOLUTION; BACTERIAL cell surfaces; NUCLEOTIDE sequencing; RESEARCH; EVALUATION research; COMPARATIVE studies; GRAM-negative aerobic bacteria; STUDENTS; SCHOOLS; RESEARCH funding; CARRIER state (Communicable diseases); MEMBRANE proteins; INFECTIOUS disease transmission
- Publication
Journal of Infectious Diseases, 2021, Vol 223, Issue 12, p2038
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiaa674