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- Title
Adipose tissue adaptive response to trans-10,cis-12-conjugated linoleic acid engages alternatively activated M2 macrophages.
- Authors
Pini, Maria; Touch, Sothea; Poirier, Hélène; Dalmas, Elise; Niot, Isabelle; Rouault, Christine; Druart, Céline; Delzenne, Nathalie; Clément, Karine; André, Sébastien; Guerre-Millo, Michèle
- Abstract
In mice, nutritional supplementation with the trans-10,cis-12 isomer of linoleic acid (t10,c12-CLA) promotes lipoatrophy, hyperinsulinemia, and macrophage infiltration in white adipose tissue (WAT). We explored the dynamics of these interrelated responses over 2 consecutive 7 d periods of t10,c12-CLA administration and withdrawal. t10,c12-CLA down-regulated lipogenic and lipolytic gene expression and increased collagen deposition, but with no evidence of cross-linking. An abundant CD45+ cell infiltrate, comprising prominently CD206+CD11c- macrophages, was found in WAT in association with an anti-inflammatory gene signature. Infiltration of natural killer (NK) and dendritic cells contributed to WAT's innate immune response to t10,c12-CLA. Less abundant adaptive immune cells colonized WAT, including B, NK T, ?d T, and aβ T cells. By contrast, T-regulatory cell abundance was not affected. Interruption of treatment allowed recovery of WAT mass and normalization of insulinemia, coincident with regain of WAT homeostasis owing to a coordinated reversion of genic, structural, and immune deregulations. These data revealed a striking resilience of WAT after a short-term metabolic injury induced by t10,c12-CLA, which relies on alternatively activated M2 macrophage engagement. In addition, the temporal links between variations in WAT alterations and insulinemia upon t10,c12-CLA manipulation strengthen the view that WAT dysfunctional status is critically involved in altered glucose homeostasis.--Pini, M., Touch, S., Poirier, H., Dalmas, E., Niot, I., Rouault, C., Druart, C., Delzenne, N., Clément, K., André, S., Guerre-Millo, M. Adipose tissue adaptive response to trans-10,cis-12-conjugated linoleic acid engages alternatively activated M2 macrophages.
- Subjects
ADIPOSE tissue physiology; LINOLEIC acid; MACROPHAGE activation; CIS-trans isomers; KILLER cells; HYPERINSULINISM; METABOLIC regulation
- Publication
FASEB Journal, 2016, Vol 30, Issue 1, p241
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.15-276675